CPT1A anticorps (AA 122-556)

N° du produit ABIN7599853

Cet anticorps anti-CPT1A est un anticorps Lapin Polyclonal détectant CPT1A dans WB, IHC, ELISA, ICC, IF et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour CPT1A anticorps (AA 122-556) (ABIN7599853)

Antigène: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPT1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-CPT1A anticorps

Épitope: AA 122-556

Fonction: Anti-CPT1A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-CPT1A Antibody Picoband® (ABIN7599853). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CPT1A recombinant protein (Position: M122-K556).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Andersen, M. K., Hansen, T. Genetics of metabolic traits in Greenlanders: lessons from an isolated population. J. Intern. Med. 284: 464-477, 2018. 2. Andersen, M. K., Jorsboe, E., Sandholt, C. H., Grarup, N., Jorgensen, M. E., Faergeman, N. J., Bjerregaard, P., Pedersen, O., Moltke, I., Hansen, T., Albrechtsen, A. Identification of novel genetic determinants of erythrocyte membrane fatty acid composition among Greenlanders. PLoS Genet. 12: e1006119, 2016. Note: Electronic Article. 3. Bergstrom, J. P., Reitz, R. C. Studies on carnitine palmitoyl transferase: the similar nature of CPTi (inner form) and CPTo (outer form). Arch. Biochem. Biophys. 204: 71-78, 1980.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur CPT1A

Antigène: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Autre désignation: CPT1A

Sujet:

Synonyms: CD59A glycoprotein, MAC-inhibitory protein, MAC-IP, Membrane attack complex inhibition factor, MACIF, Protectin, CD59, Cd59a, Cd59

Tissue Specificity: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.

Background: The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 88 kDa

ID gène: 1374

UniProt: P50416

Pathways: AMPK Signaling, Regulation of Lipid Metabolism by PPARalpha, Feeding Behaviour, Monocarboxylic Acid Catabolic Process